STRESS, INFERTILITY AND HERPES INFECTION
Mary C. Wakeman, D.V.M.
Ashford Animal Clinic
Canine Fertility Center
Ashford, CT

Our most frequent discussions about Herpes in dogs occur because of neonatal puppy death. Herpes causes puppies to die in the first two to three weeks of life, when puppies cannot regulate their body temperature. After three weeks of age the puppies acquire the ability to regulate their body temperature, and to mount a fever response. At that time, the disease no longer causes death, but can cause a respiratory disease.

We rarely find much in references about this disease in the adult dog. Where it is mentioned, it is generally in regard to future breeding advisability for a bitch that has produced a litter of puppies that had herpes, or regarding the presence of vesicles (blister like lesions) on the vulva or prepuce. Recent papers published by Dr. James Everman of Washington State University, have demonstrated that the disease as we know it in dogs is very similar to the equivalent disease in horses (Rhino). This information fills in the remainder of the picture for dogs in a very dramatic fashion.

Those of us who have owned horses are likely to be familiar with the equivalent Herpes virus infection in horses, a disease called Rhino (Rhinopneumonitis). Rhino in horses is characterized by a mild respiratory disease in most cases, and by abortion in pregnant mares. In the respiratory form of the disease, the horses have a clear nasal discharge, may snort or cough, and have reduced exercise tolerance. It is a mild condition, and unless the horse is being actively used or campaigned, or is pregnant, it is rarely vaccinated against. The vaccine, somewhat like the common cold virus, does not give a good duration of immunity, and if protection from Rhino is desired, it has been necessary to vaccinate every 4 months. Rhino in horses also causes abortion. On breeding farms, an epidemic of Rhino will cause what is called an "abortion storm" in brood-mares. That frightening phrase says it all. Rhino is highly contagious, spread through the air by aerosols or contact with secretions.

The fact that Herpes virus infection in dogs has been given such little attention by veterinarians, when its effects might well have anticipated by reproductive experts, serves as an excellent demonstration of the historical lack of interest in canine reproduction by the Veterinary establishment in general.

The respiratory form of Herpes infection in adult dogs is minimal in its signs. It is, however, extremely important in the transmission of the disease. It is not necessary for dogs to acquire Herpes as a venereal disease. A dog may acquire Herpes as an airborne aerosol, or from aerosols on your clothing, for instance, and abort or resorb a litter. I have personally heard of only two cases of adult dogs that were sneezing, in the manner of a human with a cold. Any dog may sneeze once or twice in response to inhaling an irritant. But sneezing consistently, over a protracted period, is extremely rare.

One instance of frank sneezing occurred in 6 week old puppies, who were housed near another litter in the same household. This litter was 3.5 weeks older than the younger litter. The older litter had Herpes in the respiratory form with sneezing and clear nasal secretions at 5 to 6 weeks, while the younger ones, under 3 weeks of age, had suffered a 50% mortality and were confirmed as having Herpes on post mortem examination.

The older puppies who were affected only with the sneezing were able to regulate their body temperature. Puppies die of Herpes only so long as they are unable to mount a fever response. Between 2 and 3 weeks puppies develop the ability to control their temperature. Earlier they are dependant on their surroundings for warmth -- this is called poikilothermia. Treatment of young puppies where herpes has been diagnosed by a postmorten exam of one of the dead puppies involves elevating the temperature of the surroundings. Check with your veterinarian if you think this may be the problem in your litter under three weeks of age. Refrigerate (do not freeze) dead puppies so they can be taken in for post mortem examination.

As Herpes is a virus, there is no particular reason for administering antibiotics, unless a secondary bacterial infections occurs. If there is uncertainty as to whether Herpes or a neonatal bacterial infection is causing puppy death, antibiotics are appropriate. A cervical culture and sensitivity of the bitch prior to whelping to determine the presence of E. coli will provide help in making this decision, since E.coli also causes neonatal death.

Litter loss can occur at any point of the pregnancy. Puppies may be resorbed or born dead, or they may acquire Herpes virus as they pass through the vaginal canal at birth (along with E.coli bacteria), in utero prior to birth, or by contact with infective secretions (respiratory aerosols or vaginal discharges) after birth. Bitches infected with Herpes prior to breeding or from exposure at the time of breeding simply never show as pregnant. The best way of preventing gestational loss or neonatal death is to quarantine the dam from 3 weeks prior to breeding to 3 weeks after whelping. To determine if a bitch has been freshly exposed to Herpes during gestation, and has not whelped for that reason, 2 serum samples, taken 2 weeks apart, are submitted to a lab for a Herpes titer. A changing titer (either rising or falling) indicates that Herpes infection may have caused the loss of a litter in gestation.

Herpes infection of the dam which does not result in abortion may result in low birth weight, weak or stillborn puppies. If there are surviving puppies in a Herpes litter, they may or may not have permanent damage to the nervous system, kidney, or lymphoid tissue, much in the way that puppy strangles may damage internal organs and cause problems later in life.

Once we have experienced Herpes in a litter of neonates, we are critically interested in whether or not we are likely to experience a repeat occurrence if we breed the dam again. We are also interested in whether the stud dog must be infected. Herpes in adult dogs tends to go dormant, as it does in humans, and to resurface and become acute in times of stress. Consider the stresses in play for the bitch being bred:

Hormonal events which temporarily impair the body’s ability to fight infection.
Shipping, boarding, breeding and shipping again, should the bitch travel to the stud dog.
Possible concurrent bacterial vaginal or uterine disease:
In appropriate parts of the country, concurrent lyme disease.
Showing.
In the bitch or 5 years or older, decreased thyroid levels.
Any other stresses, such as temperature stress.
Knowing that stress may activate Herpes, (which may then in 3 weeks cause early embryonic death) goes a very long way toward explaining those bitches we have been so certain were bred up to the fourth week, but failed to whelp at term, or that whelped a much smaller than anticipated litter. Where we have done thorough pre-breeding workups and have palpated normal vesicles in a bitch at 28 days of gestation, yet no puppies were delivered, we test for Herpes with paired serum samples taken 2 weeks apart, at about the time the bitch should have whelped. Where the bitch was in good health and clean of bacterial vaginal or uterine disease originally, had normal vesicles and later lost her litter, we have found positive Herpes titers in about 80% of cases. This should be an extremely valuable piece of information for breeders, especially those who show regularly, have multiple animals in their kennel, who groom or run boarding kennels, or who give obedience lessons, and so on. Remember, this disease can literally be carried home on your clothing.

What is the expectation for a bitch which has whelped a known Herpes litter (diagnosed by post mortem exam), or has been shown by titers to have lost a litter in gestation due to Herpes? If she is stressed by some of the factors above, she may be affected again. On the other hand, if she is bred at home or driven back and forth to the stud, given a complete pre-breeding medical workup, including thyroid testing, blood count, and cervical culture and sensitivity, and has any detrimental conditions cleared up, she is perfectly likely to have no problem. This also supposes that she is not traveled around during her gestation and taken to shows, that she is kept apart from those dogs coming into the kennel from outside and those traveling to shows, and so forth. It also includes not going to classes where there are other dogs as well. In a breed felt to be immunodeficient (Rottweilers, Dobermans, Boxers and possibly Pugs, Collies, and Shelties) the risk of a repeat occurrence will be greater.

If stresses are avoided, most bitches have no problem in subsequent breedings.
Nearly all dogs with any doggy contacts at all have been exposed to Herpes. It is not just or primarily a venereal disease. It is not usually or necessarily contracted through the breeding. It is usually contracted through exposure to the respiratory aerosols of other dogs. The very short-term immunity a new exposure may confer fades quickly, leaving the dog open to repeat infection as well as to dormant infections re-surfacing. In fact, it would be very hard to say that any repeat of the infection came from a dormant infection instead of a new exposure to high levels of infective particles. Just going to the stud dog’s premises can be enough to expose the bitch; if there are frequent visiting bitches to the kennel, new infections may be brought with them. If the stud dog or his kennel mates are showing or training, he may be shedding high levels of particles. It is simply not possible to avoid this virus in the dog’s environment. But it is possible to limit that exposure; if going to the stud dog, don’t travel by air, and stay in a motel with the bitch rather than leaving her at the stud’s kennel. If going to a dog show, completely change clothes and wash before encountering any bred bitches at home. If training or showing kennel mates, isolate her from them and perform her ‘chores’ before doing theirs, always going from "clean to dirty".

The most susceptible bitch of all, however, will be the one that lives in a pet home and has not had routine exposure to other dogs or the virus. Such a bitch upon being exposed to the virus will be more seriously affected that a bitch with routine exposure to low levels of the virus.

A stud dog bred to an infected bitch may or may not be infected, either as the animal which passed the infection to the bitch or as a recipient of an acute infection she was harboring when he bred her. Herpes testing at the time of breeding is meaningless, since almost all individuals in the bitch’s environment may be in various phases of acquiring or recovering from infection. It bears repeating here, that the respiratory infection is very mild and rarely ever shows actual clinical signs in adult dogs. Only paired serum samples taken at different times to detect a rising (becoming infected ) titer, or a falling ( recovering from an infection) titer, can tell us anything. Obviously the information generated in this way will be too late to be of use at the time of breeding. The usefulness of these paired titers is seen chiefly as a tool to identify if a bitch has lost her litter due to Herpes after the fact. We can rule Herpes in or out as one of the several options for causes of litter resorbtion, or still born or sickly neonates.

The best rule to follow is that a kennel with an active show schedule, a stud dog which is being shown or trained, a boarding kennel, or a kennel where breedings are frequent, so that visiting bitches are frequently on the premises is likely to harbor infective particles. The worst shedders however, will be pet dogs newly exposed to a heavily trafficed environment, such as a boarding kennel. These dogs will be more seriously affected and will shed more virus particles. A visiting bitch staying in a kennel where susceptible pets are passing through will likely be more at risk than in visiting a show kennel, where all the dogs will have some resistance at all times, and shedding will be at lower levels.

Certainly fighting a uterine or vaginal infection may be stress a bitch sufficiently to produce an adverse affect on resistance to Herpes. Certainly any bitch low in thyroid would find it more difficult to resist an infection. The thyroid levels necessary for the life of a comfortable pet and those necessary for reproduction, especially in the bitch, are not the same. Nature wants our children (human) to have children as teen agers, not as 40 year olds. We, of course don’t feel quite the same about children reproducing. Likewise, Nature wants dogs to whelp at 18 months, not 6 years of age. Thyroid normals represent all breeds, all ages, and all states of health. It has been said that all dogs over 5 years old show a decrease in their thyroid level - they’re starting to age. To lump the thyroid samples of 6 to 12 year olds with those of 1 to 5 year olds throws the ‘normal range’ off considerably. Probably a good statement to make is that to ensure that thyroid doesn’t contribute to infertility, it should be (T4) in the upper one third to one fourth of the normal range. When looked at in a biologic perspective, when thyroid hormone decreases, naturally the first considerations of the body are to maintain health in the individual. Only in animals who are young and healthy enough to nurture puppies will Nature encourage reproduction, as a matter of course. On the other hand, since we have created most of our breeds in the last 100 to 200 years, it is somewhat unrealistic for us, with the kinds of remedies we have at hand, to worry overly much about what Nature wants of one of our dogs. If a bitch is in good health and over 5, and simply needs supplementation of l-thyroxin to continue to reproduce, it would be silly of us to worry about what Nature wants. Our dogs don’t live in ‘Nature’ - except perhaps those running the Iditarod. A given animal’s suitability for breeding should be determined on its thyroid as a young dog, not on its levels in when it’s over 5 years of age.

Other sources of immune suppression are parvo disease, lyme disease, and vaccinations with your regular ‘booster’ vaccine which contains Distemper and Hepatitis (A2) virus, and which because of the combination of these two together causes about a 2 week immunosuppression. Parvo, like Herpes is endemic in our dog show population, and may be brought home from a show, and cycled by other dogs in the kennel subclinically, later affecting both the bred bitch and the young puppies in a way that it wouldn’t affect the rest of the adults in the kennel. Drugs (corticosteroids, chemotherapy), emotional family situations, moving, and so on can all produce stress.

One of the chief uses of ‘chilled’ or ‘fresh-extended’ semen is to avoid the stresses of travel to breed a bitch. All of the common sense cautions we used to be aware of – don’t take your pregnant bitch to shows, and don’t ship her to be bred – are certainly largely explained by the conditions fostering a Herpes infection, resulting in early embryonic loss, resorbtion, still birth, weak puppies, and neonatal death.

Herpes is one of the very few things that any veterinarian can determine on a quick autopsy of a dead puppy. There is grossly visible mottling on the kidneys that is totally diagnostic for Herpes. If you have a puppy die, refrigerate the body (don’t freeze it) and get your vet to look at it ASAP. Then ask the vet for directions for raising the body temperature of the remaining puppies in the litter.

 

 

Canine Herpesvirus Infection

by Race Foster, DVM and Marty Smith, DVM
Drs. Foster & Smith, Inc.

Canine herpesvirus is generally referred to as CHV, and is a leading cause of puppy deaths, especially in puppies one to three weeks of age. We have all heard of breeders saying something like this—"The puppies were fine this morning, but then they stopped eating and died before I could do anything!" Anytime puppies die in this fashion, there is a reason to suspect CHV.

Transmission
Canine herpesvirus is a viral disease that affects many puppies, causing sporadic deaths and occasionally the death of an entire litter. The virus lives in the reproductive and respiratory tracts of male and female dogs and can be sexually transmitted. The virus persists in the female's vaginal secretions and the male's semen. As in many herpes infections found in other species, adult animals can live for years with no apparent signs; these are called "asymptomatic carriers". This means the adult male and female dogs can remain infected and transmit the disease for years while showing no signs of disease themselves.

Puppies can become infected several ways. The virus can cross the placenta and infect them while they are still within the uterus, or they may become exposed from vaginal secretions during birth. The virus can also become airborne from nasal secretions of the mother, so once born, the pup can actually inhale the virus while breathing. Puppies can easily spread the virus from one to another. Lastly, the virus can be transmitted by eating infected materials.

Symptoms
Once exposed, it generally takes about a week for symptoms to appear. With this in mind, you can easily see why 1 to 3 week old puppies are at the highest risk. Severely infected individuals will become depressed, stop nursing and cry. Their feces will be soft and yellow-green. Their livers enlarge and their abdomens are painful. The liver becomes damaged and can no longer function normally. Some puppies develop respiratory signs and nasal discharge. Others develop a rash on their abdomen. Hemorrhages such as nose bleeds and small bruises on the mucous membranes or skin may appear. Some puppies will show nervous system signs such as blindness and staggering. Puppies usually die within 24-48 hours of showing signs of disease.

Not all pups exposed at birth become ill, and many show no signs at all or develop only a slight congestion and recover within a few days.Puppies exposed after six weeks of age have a better chance of recovery. Older puppies develop the disease by coming in contact with the mother's infected, but normal-appearing, nasal secretions.Those that live often develop into carrier adults just like their parents.

It appears that the virus thrives best at a temperature of around 99°F, so this may help to explain why older puppies are at less risk as their body temperature is usually around 101.5°F.

Adult carriers typically exhibit no obvious symptoms, however, small blister-like lesions may occasionally be noted on the vaginal wall.

Making a diagnosis
Canine herpesvirus is one of the leading causes of death in newborn puppies. Once the above signs develop, death often follows in 48 hours. The disease spreads rapidly through the litter as infected puppies are highly contagious. We suspect many cases of herpes are wrongly diagnosed as disorders such as parvovirus and coronavirus. Autopsies of deceased puppies by a veterinary pathologist will reveal the characteristic herpes lesions.

A final diagnosis can only be made following an autopsy. Diagnostic hemorrhagic lesions will be found within the kidney and liver, and the lungs will usually be congested. The affected organs will have cells containing characteristic signs of the disease.

Treatment and prevention
Currently there is no treatment for canine herpes. It is hoped that a vaccination will be developed to protect against this disease.

Unfortunately, there is no treatment. All treatment is aimed at supportive care. Older puppies can be force-fed and treated with anti-diarrheal medication. The survival rate of puppies less than three weeks old is poor. Severely infected puppies will die rapidly. The remaining puppies should be kept warm (100°F) until the virus has run its course. No vaccine is available.

As a preventive practice, one should make sure the environmental temperature of the puppies is kept warm with whelping boxes, heat lamps, etc.

In conclusion, anytime one sees upper respiratory infections in puppies less than 8 weeks of age, CHV should be considered. Also, CHV is a leading cause of death in 1 to 3 week old pups, and even older puppies may die. The deaths will occur suddenly, with little or no warning, and one pup or even an entire litter may perish within a 24-hour period. This is especially disheartening when yesterday the litter appeared happy and healthy, and today some are ill or have already died. Remember, the mother may be a carrier and future litters may be affected unless she develops a natural immunity. There is no treatment for her or for the puppies.

Disclaimer: This article is intended for educational purposes only. All content is general pet health information and not specific to your pet. You should not, therefore, rely solely on the information, products or services mentioned in the article in lieu of consultation with your own veterinarian.